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Address: 14th Floor, Building A, Wuhan Guoying Seed Industry Building, No. 666, Shendun Fourth Road, Jiangxia District, Wuhan, Hubei Province
Email: pinuofei2017@163.com
TEL: 15392937510
Article Number: PK0117
Delivery time: 现货
Price: 50 μL/960; 100 μL/1600; 200 μL/2560
Category:
Target： NFKB1
Application： WB, ELISA, IHC
Reactivity ： Human,Mouse
MW(Observed) ： 50 kDa, 106 kDa
Host Species： Rabbit
Isotype ： IgG
隐藏域元素占位
Detailed Information
Recommended dilution ratio ： WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000
Compose ： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification process ： The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Storage ： -15°C to -25°C/1 year(Do not lower than -25°C
Concentration ： 1 mg/ml
Clonality ： Polyclonal
Antigen & Target Information
Specificity ： This antibody detects endogenous levels of Human,Mouse NF-κB p105 (Cleaved-Thr434, protein was cleaved amino acid sequence between 433-434 )
Gene name ： NFKB1
Protein Name ： NF-κB p105 (Cleaved-Thr434)
Alias ： Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; [Cleaved into: Nuclear factor NF-kappa-B p50 subunit]
Background：
domain:Glycine-rich region (GRR) appears to be a critical element in the generation of p50.,domain:The C-terminus of p105 might be involved in cytoplasmic retention, inhibition of DNA-binding, and transcription activation.,function:NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL, and NFKB2/p52, with the heterodimeric p65-p50 complex appearing to be the most abundant form. These dimers bind to kappa-B sites in the DNA of their target genes, and individual dimers exhibit distinct preferences for different kappa-B sites, binding with varying affinities and specificities. Different dimer combinations function as either transcriptional activators or repressors. NF-kappa-B is regulated through various mechanisms, including post-translational modifications, subcellular compartmentalization, and interactions with other cofactors or corepressors. NF-kappa-B complexes are maintained in the cytoplasm in an inactive state, bound to members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to various activators, subsequently leading to its degradation and release of the active NF-kappa-B complex, which then translocates into the nucleus. The NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes serve as transcriptional activators. The NF-kappa-B p50-p50 homodimer acts as a transcriptional repressor but can function as an activator when associated with BCL3. NFKB1 appears to have dual roles: it mediates cytoplasmic retention of attached NF-kappa-B proteins via p105 and generates p50 through cotranslational processing. A proteasome-mediated process ensures the production of both p50 and p105 while preserving their independent functions, although post-translational processing of NFKB1/p105 also seems to occur. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer regions of genes involved in immune responses and acute-phase reactions. In complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released through proteasome-dependent degradation of NFKB1/p105.,induction:By phorbol ester and TNF-alpha.,PTM:Phosphorylation at 'Ser-903' and 'Ser-907' primes p105 for proteolytic processing in response to TNF-alpha stimulation. Phosphorylation at 'Ser-927' and 'Ser-932' is required for BTRC/BTRCP-mediated proteolysis.,PTM:Polyubiquitination seems to facilitate p105 processing.,PTM:S-nitrosylation of Cys-61 affects DNA binding.,PTM:While translation occurs, the unique unfolded structure following the GRR repeat promotes the generation of p50, making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active, whereas the unprocessed form acts as an inhibitor (I-kappa-B-like), capable of forming cytosolic complexes with NF-kappa-B and trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes further processing.,similarity:Contains 1 death domain.,similarity:Contains 1 RHD (Rel-like) domain.,similarity:Contains 7 ANK repeats.,subcellular location:Nuclear, but also found in the cytoplasm in an inactive form complexed with an inhibitor (I-kappa-B).,subunit:Component of the NF-kappa-B p65-p50 complex. Component of the NF-kappa-B p65-p50 complex. Homodimer; component of the NF-kappa-B p50-p50 complex. Component of the NF-kappa-B p105-p50 complex. Component of the NF-kappa-B p50-c-Rel complex. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Also interacts with MAP3K8. NF-kappa-B p50 subunit interacts with NCOA3 coactivator, which may coactivate NF-kappa-B–dependent expression via its histone acetyltransferase activity. Interacts with DSIPI; this interaction prevents nuclear translocation and DNA-binding. Interacts with SPAG9 and UNC5CL. NFKB1/p105 interacts with CFLAR; this interaction inhibits p105 processing into p50. NFKB1/p105 forms a ternary complex with MAP3K8 and TNIP2. Interacts with GSK3B; this interaction prevents processing of p105 into p50. NFKB1/p50 interacts with NFKBIE. NFKB1/p50 interacts with NFKBIZ. Nuclear factor NF-kappa-B p50 subunit interacts with NFKBID.,
Cell localization ： Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
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Address: 14th Floor, Building A, Wuhan Guoying Seed Industry Building, No. 666, Shendun Fourth Road, Jiangxia District, Wuhan, Hubei Province
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