Product Services
Signaling pathway
-
-
-
Neurodegeneration—A Common Pathway Across Multiple Diseases (0)
-
The AGE–RAGE signaling pathway in diabetic complications (0)
-
PD-L1 Expression and the PD-1 Checkpoint Pathway in Cancer (0)
-
Epithelial Cell Signaling in Helicobacter pylori Infection (0)
-
Glioma-associated Kaposi’s sarcoma-associated herpesvirus infection (0)
Contact Us
Address: 14th Floor, Building A, Wuhan Guoying Seed Industry Building, No. 666, Shendun Fourth Road, Jiangxia District, Wuhan, Hubei Province
Email: pinuofei2017@163.com
TEL: 15392937510
Article Number: PL0544
Delivery time: 现货
Price: 50 μL/960; 100 μL/1600; 200 μL/2560
Target： Topoisomerase I
Application： WB, IHC, IF, ELISA
Reactivity ： Human,Mouse,Rat
MW(CalcμLated) ： 91kD
MW(Observed) ： 91kD
Host Species： Rabbit
Isotype ： IgG, Kappa
隐藏域元素占位
Detailed Information
Recommended dilution ratio ： IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000;
Compose ： PBS, 50% glycerol, 0.05% Proclin 300, 0.05% BSA
Purification process ： Protein A
Storage ： -15°C to -25°C/1 year(Do not lower than -25°C
Clonality ： Monoclonal
Clone Number ： PT1218R
Antigen & Target Information
Specificity ： Endogenous
Gene name ： TOP1
Protein Name ： DNA topoisomerase 1
Alias ： TOP1;DNA topoisomerase 1;DNA topoisomerase I
Database connection:
Background：
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008],
Cell localization ： Catalytic activity: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. Disease: A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. enzyme regulation: Specifically inhibited by camptothecin (CPT), a plant alkaloid with antitumor activity. Function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. miscellaneous: Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils. miscellaneous: When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand. PTM: Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment. similarity: Belongs to the eukaryotic type I topoisomerase family. subcellular location: Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumoylated forms found in both nucleoplasm and nucleoli. subunit: Monomer. Interacts with SV40 Large T antigen; this interactions allows viral DNA replication.
Recommended
Relevant Literature
We remain open to communication and look forward to supporting you.
Centered on our primary and secondary antibody products, we have established a strong foothold in the specialized antibody reagent market. Leveraging our independently developed core technology platform and a dedicated R&D and manufacturing team, we provide high‑reliability biological reagents and end‑to‑end technical solutions to universities, research institutes, biopharmaceutical companies, and other customers worldwide.
Support Services. Since its inception, Pinofibio has remained committed to a development philosophy centered on research needs. Through a robust R&D system, stringent quality control, and responsive, efficient service, the company is dedicated to building an antibody product portfolio that spans multiple species and targets, helping researchers overcome technical challenges and driving innovation in the biopharmaceutical industry.
Wuhan Pinofibio Biotechnology Co., Ltd.
Address: 14th Floor, Building A, Wuhan Guoying Seed Industry Building, No. 666, Shendun Fourth Road, Jiangxia District, Wuhan, Hubei Province
Email: pinuofei2017@163.com
TEL: 15392937510
Xiaohongshu
Experimental Services
Official Account
Antibody Services
微信客服