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Address: 14th Floor, Building A, Wuhan Guoying Seed Industry Building, No. 666, Shendun Fourth Road, Jiangxia District, Wuhan, Hubei Province
Email: pinuofei2017@163.com
TEL: 15392937510
Article Number: PH0992
Delivery time: 两周
Price: 100 μL/2200
Target： PCNA
Application： WB, IF, IHC
Reactivity ： Human, Rat, Mouse
MW(Observed) ： 30-33 kDa
Host Species： Mouse
Isotype ： IgG1
Conjugate ： AbFluor 594
隐藏域元素占位
Detailed Information
Recommended dilution ratio ： Optimal working dilutions should be determined experimentally by the investigator;Suggested starting dilutions are as follows:IHC 1:200;IF 1:200.
Compose ： Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.
Purification process ： The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Storage ： Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.
Concentration ： 1 mg/mL
Clonality ： Monoclonal
Clone Number ： 12D10
Antigen & Target Information
Specificity ： PCNA Monoclonal Antibody(12D10) AbFluor™ 594 Conjugated specially designed for your Immunofluorescence analysis.
Gene name ： PCNA
Protein Name ： Proliferating cell nuclear antigen
Alias ： PCNA; Proliferating cell nuclear antigen; PCNA; Cyclin
Background：
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008],
Cell localization ： Nucleus. Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136). Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136). Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. .
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Wuhan Pinofibio Biotechnology Co., Ltd.
Address: 14th Floor, Building A, Wuhan Guoying Seed Industry Building, No. 666, Shendun Fourth Road, Jiangxia District, Wuhan, Hubei Province
Email: pinuofei2017@163.com
TEL: 15392937510
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