Signaling pathway

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WT1 Polyclonal Antibody

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Article Number: PG6464
Delivery time: 现货
Price: 50 μL/960; 100 μL/1600; 200 μL/2560
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Target: Wilms' Tumor 1
Application: WB, ELISA
Reactivity : Human, Rat, Mouse
MW(Observed) : 49 kD
Host Species: Rabbit
Isotype : IgG

隐藏域元素占位

Detailed Information

Recommended dilution ratio : WB 1:500-2000; ELISA 1:5000-20000
Compose : PBS, 50% glycerol, 0.05% Proclin 300, 0.05% BSA
Purification process : The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage : -15°C to -25°C/1 year(Do not lower than -25°C
Concentration : 1 mg/ml
Clonality : Polyclonal

Antigen & Target Information

Specificity : WT1 Polyclonal Antibody detects endogenous levels of protein.
Gene name : WT1
Protein Name : Wilms tumor protein (WT33)

Database connection:

Organism

Gene ID

SwissProt

Mouse
Background:

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015],

Cell localization : Nucleus . Nucleus, nucleolus. Cytoplasm . Isoforms lacking the KTS motif have a diffuse nuclear location (PubMed:15520190). Shuttles between nucleus and cytoplasm. .; [Isoform 1]: Nuclear speckle .; [Isoform 4]: Nucleus, nucleoplasm .

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