Signaling pathway

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PRAK Monoclonal Antibody

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Article Number: PH1488
Delivery time: 一周
Price: 100 μL/2200
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Target: MAPKAPK5
Application: WB, IHC, IF, ELISA
Reactivity : Human
MW(CalcμLated) : 54 kD
Host Species: Mouse

隐藏域元素占位

Detailed Information

Recommended dilution ratio : WB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000; IF 1:50-200
Compose : Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification process : Affinity purification
Storage : -15°C to -25°C/1 year(Do not lower than -25°C
Clonality : Monoclonal

Antigen & Target Information

Specificity : PRAK Monoclonal Antibody detects endogenous levels of PRAK protein.
Gene name : MAPKAPK5
Protein Name : MAP kinase-activated protein kinase 5
Alias : MAPKAPK5;PRAK;MAP kinase-activated protein kinase 5;MAPK-activated protein kinase 5;MAPKAP kinase 5;MAPKAP-K5;MAPKAPK-5;MK-5;MK5;p38-regulated/activated protein kinase;PRAK

Database connection:

Organism

Gene ID

SwissProt

Mouse
Background:

The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012],

Cell localization : Cytoplasm. Nucleus. Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export.

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