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Address: 14th Floor, Building A, Wuhan Guoying Seed Industry Building, No. 666, Shendun Fourth Road, Jiangxia District, Wuhan, Hubei Province
Email: pinuofei2017@163.com
TEL: 15392937510
Article Number: PF0728
Delivery time: 现货
Price: 50 μL/960; 100 μL/1600; 200 μL/2560
Category:
Target： MDM2
Application： IF, WB, ELISA
Reactivity ： Human, Rat, Mouse,
MW(CalcμLated) ： 55 kD
Host Species： Rabbit
Isotype ： IgG
隐藏域元素占位
Detailed Information
Modify-Modification ： Phospho
Recommended dilution ratio ： WB 1:500-2000;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.
Compose ： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification process ： The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage ： -15°C to -25°C/1 year(Do not lower than -25°C
Concentration ： 1 mg/ml
Clonality ： Polyclonal
Antigen & Target Information
Specificity ： Phospho-MDM2 (S186/S188) Polyclonal Antibody detects endogenous levels of MDM2 protein only when phosphorylated at S186/S188.
Gene name ： MDM2
Protein Name ： E3 ubiquitin-protein ligase Mdm2
Alias ： MDM2;E3 ubiquitin-protein ligase Mdm2;Double minute 2 protein;Hdm2;Oncoprotein Mdm2;p53-binding protein Mdm2
Background：
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013],
Cell localization ： Nucleus, nucleoplasm. Cytoplasm . Nucleus, nucleolus. Nucleus . Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
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Address: 14th Floor, Building A, Wuhan Guoying Seed Industry Building, No. 666, Shendun Fourth Road, Jiangxia District, Wuhan, Hubei Province
Email: pinuofei2017@163.com
TEL: 15392937510
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