Signaling pathway

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CENP-A (phospho Ser7) Polyclonal Antibody

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Article Number: PF0748
Delivery time: 现货
Price: 50 μL/960; 100 μL/1600; 200 μL/2560
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Target: CENP-A
Application: IF,ELISA
Reactivity : Human, Rat, Mouse,
MW(CalcμLated) : 16 kD
Host Species: Rabbit
Isotype : IgG

隐藏域元素占位

Detailed Information

Modify-Modification : Phospho
Recommended dilution ratio : IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications;
Compose : Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification process : The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage : -15°C to -25°C/1 year(Do not lower than -25°C
Concentration : 1 mg/ml
Clonality : Polyclonal

Antigen & Target Information

Specificity : Phospho-CENP-A (S7) Polyclonal Antibody detects endogenous levels of CENP-A protein only when phosphorylated at S7.
Gene name : CENPA
Protein Name : Histone H3-like centromeric protein A
Alias : CENPA; Histone H3-like centromeric protein A; Centromere autoantigen A; Centromere protein A; CENP-A

Database connection:

Organism

Gene ID

SwissProt

Mouse
Background:

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015],

Cell localization : Nucleus . Chromosome, centromere, kinetochore . Chromosome, centromere . Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height (PubMed:19114591). Phosphorylation at Ser-68 during early mitosis abolishes association with chromatin and centromeres and results in dispersed nuclear location (PubMed:25556658). .

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