Signaling pathway

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Bax (phospho Ser184) Polyclonal Antibody

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Article Number: PF0839
Delivery time: 现货
Price: 50 μL/960; 100 μL/1600; 200 μL/2560

Detailed Information

Modify-Modification : Phospho
Recommended dilution ratio : IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200
Compose : Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification process : The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage : -15°C to -25°C/1 year(Do not lower than -25°C
Concentration : 1 mg/ml
Clonality : Polyclonal

Antigen & Target Information

Specificity : Phospho-Bax (S184) Polyclonal Antibody detects endogenous levels of Bax protein only when phosphorylated at S184.
Gene name : BAX
Protein Name : Apoptosis regulator BAX
Alias : BAX;BCL2L4;Apoptosis regulator BAX;Bcl-2-like protein 4;Bcl2-L-4

Database connection:

Organism

Gene ID

SwissProt

Background:

The protein encoded by BAX (BCL2 associated X, apoptosis regulator) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for BAX.

Cell localization : [Isoform Alpha]: Mitochondrial outer membrane; single-pass membrane protein. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformational change that leads to its release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrial membrane. Upon Sendai virus infection, it is recruited to the mitochondrion through interaction with IRF3 (PubMed:25609812). ; [Isoform Beta]: Cytoplasm.; [Isoform Gamma]: Cytoplasm.; [Isoform Delta]: Cytoplasm.

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