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Address: 14th Floor, Building A, Wuhan Guoying Seed Industry Building, No. 666, Shendun Fourth Road, Jiangxia District, Wuhan, Hubei Province
Email: pinuofei2017@163.com
TEL: 15392937510
Article Number: PF1126
Delivery time: 现货
Price: 50 μL/960; 100 μL/1600; 200 μL/2560
Category:
Target： MSK1
Application： WB, IHC, IF, ELISA
Reactivity ： Human,Mouse
MW(Observed) ： 90 kD
Host Species： Rabbit
Isotype ： IgG
隐藏域元素占位
Detailed Information
Modify-Modification ： Phospho
Recommended dilution ratio ： WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200
Compose ： Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification process ： The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage ： -15°C to -25°C/1 year(Do not lower than -25°C
Concentration ： 1 mg/ml
Clonality ： Polyclonal
Antigen & Target Information
Specificity ： Phospho-MSK1 (S212) Polyclonal Antibody detects endogenous levels of MSK1 protein only when phosphorylated at S212.
Gene name ： RPS6KA5
Protein Name ： Ribosomal protein S6 kinase alpha-5
Alias ： RPS6KA5;MSK1;Ribosomal protein S6 kinase alpha-5;S6K-alpha-5;90 kDa ribosomal protein S6 kinase 5;Nuclear mitogen- and stress-activated protein kinase 1;RSK-like protein kinase;RSKL
Background：
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Appears to be activated by multiple phosphorylations on threonine and serine residues. ERK1/2 and MAPK14/p38-alpha may play a role in this process.,function:Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).,miscellaneous:Enzyme activity requires the presence of both kinase domains.,PTM:Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 2 protein kinase domains.,subcellular location:Predominantly nuclear. Partially cytoplasmic.,subunit:Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA5 associates with and phosphorylates the NF-kappa-B p65 subunit RELA.,tissue specificity:Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.,
Cell localization ： Nucleus. Cytoplasm. Predominantly nuclear. Exported into cytoplasm in response to glucocorticoid.
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Address: 14th Floor, Building A, Wuhan Guoying Seed Industry Building, No. 666, Shendun Fourth Road, Jiangxia District, Wuhan, Hubei Province
Email: pinuofei2017@163.com
TEL: 15392937510
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