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Address: 14th Floor, Building A, Wuhan Guoying Seed Industry Building, No. 666, Shendun Fourth Road, Jiangxia District, Wuhan, Hubei Province
Email: pinuofei2017@163.com
TEL: 15392937510
Article Number: PL0489
Delivery time: 现货
Price: 50 μL/960; 100 μL/1600; 200 μL/2560
Category:
Target： PKC θ
Application： WB, IHC, IF, ELISA
Reactivity ： Human,Mouse,Rat
MW(CalcμLated) ： 82 kD
MW(Observed) ： 79 kDa
Host Species： Rabbit
Isotype ： IgG, Kappa
隐藏域元素占位
Detailed Information
Recommended dilution ratio ： IHC 1:1000-1:2000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000;
Compose ： PBS, 50% glycerol, 0.05% Proclin 300, 0.05% BSA
Purification process ： Protein A
Storage ： -15°C to -25°C/1 year(Do not lower than -25°C
Clonality ： Monoclonal
Clone Number ： PT1273R
Antigen & Target Information
Specificity ： Endogenous
Gene name ： PRKCQ
Protein Name ： Protein kinase C theta type
Alias ： PRKCQ;PRKCT;Protein kinase C theta type;nPKC-theta
Background：
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008],
Cell localization ： Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Domain: The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor, and the C2 domain is a non-calcium-binding domain. Enzyme regulation: Three specific sites—Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif), and Ser-695 (hydrophobic region)—must be phosphorylated for full activation. Function: PKC is activated by diacylglycerol, which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Function: This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PTM: Autophosphorylation at Thr-219 is required for targeting to the TCR and for PKC’s cellular function upon antigen receptor ligation. Similarity: Belongs to the protein kinase superfamily. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Similarity: Contains 1 AGC-kinase C-terminal domain. Similarity: Contains 1 C2 domain. Similarity: Contains 1 protein kinase domain. Similarity: Contains 2 phorbol-ester/DAG-type zinc fingers. Subunit: Interacts with TXNL2/PICOT. Tissue specificity: Skeletal muscle, megakaryoblastic cells, and platelets.
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Email: pinuofei2017@163.com
TEL: 15392937510
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